Paciente con malformación de Arnold Chiari en programa de cirugía ambulatoria / No disponible

Introducción: La malformación de Arnold Chiari es relativamente frecuente; este hecho, combinado a un aumento de los procesos quirúrgicos ambulatorios, implica la necesidad de establecer una estrategia para la anestesia de tipo ambulatorio. Caso clínico: Presentamos el caso de un paciente de 61 años, con antecedente de malformación de Arnold Chiari tipo I programado para herniorrafia inguinal en cirugía mayor ambulatoria. El perioperatorio cursa sin complicaciones, con una recuperación satisfactoria cumpliendo los objetivos de la cirugía mayor ambulatoria. Hemos aplicado medidas para prevenir complicaciones relacionadas como la hipertensión intracraneal e inestabilidad hemodinámica, y nos hemos preparado para afrontar una posible vía aérea difícil. Conclusiones: Los pacientes con diagnóstico de malformación de Arnold Chiari tipo I asintomáticos, pueden beneficiarse de los programas de cirugía sin ingreso, el éxito depende del adecuado manejo perioperatorio (AU)

Introduction: The Arnold Chiari malformation is a relatively common disease; this fact, combined with an increase in outpatient surgical procedures, involves the need to establish a strategy for outpatient anesthesia. Case report: We report a 61-year-old man with a history of Arnold Chiari malformation type I schedule for inguinal hernia repair in ambulatory surgery. The perioperative course was uncomplicated with satisfactory recovery to meet the objectives of the ambulatory surgery. We have implemented measures to prevent complications such as intracranial hypertension and hemodiynamicinstability, and we prepared for a possible difficult airway. Conclusions: Patients diagnosed with Arnold Chiari malformation type I asymptomatic, may befit from outpatient surgery programs, success depends on the appropriate perioperative management (AU)

Growth efficiency in transgenic tilapia (Oreochromis sp.) carrying a single copy of an homologous cDNA growth hormone.

Growth hormone (GH) has been shown to have a profound impact on fish physiology and metabolism. However, detailed studies in transgenic fish have not been conducted. We have characterized the food conversion efficiency, protein profile, and biochemical correlates of growth rate in transgenic tilapia expressing the tilapia GH cDNA under the control of human cytomegalovirus regulatory sequences. Transgenic tilapia exhibited about 3.6-fold less food consumption than nontransgenic controls (P < 0.001). The food conversion efficiency was significantly (P < 0.05) higher (290%) in transgenic tilapia (2.3 +/- 0.4) than in the control group (0.8 +/- 0.2). Efficiency of growth, synthesis retention, anabolic stimulation, and average protein synthesis were higher in transgenic than in nontransgenic tilapia. Distinctive metabolic differences were found in transgenic juvenile tilapia. We had found differences in hepatic glucose, and in agreement with previous results we observed differences in the level of enzymatic activities in target organs. We conclude that GH-transgenic juvenile tilapia show altered physiological and metabolic conditions and are biologically more efficient.

[New protocol for the early diagnosis of acoustic schwannoma in the outpatient clinic]. / Nuevo protocolo para el diagnóstico precoz del neurinoma del acústico en consulta ambulatoria.

For the last two years we have been testing a protocol for early diagnosis of acoustic schwannoma. We analyzed the results obtained in outpatients of the ENT clinic for ten months. Using the protocol described 10.7% accurate diagnoses, were obtained although in only one diagnosis was made in the intracanalicular stadium that was susceptible to ENT surgery.

Applications of monoclonal antibodies in aquaculture.

Monoclonal antibodies (MAbs) currently are being applied to the study of fish immunology and fish infectious diseases. MAbs to fish immunoglobulins (Igs) have helped isolate fish Igs, identify heavy and light chain variants in fish Ig, study the ontogeny of B lymphocytes, and improve techniques for the measurement of fish Ig and specific antibodies (Abs). MAbs have been obtained against several leucocyte surface antigens and are being used as markers for different subsets of fish leukocytes: neutrophils, non-specific cytotoxic cells and cells responsible for the mixed leucocyte reaction. The sensitivity and specificity of many immunoassays for identifying fish pathogens have been improved by the use of MAbs. Further improvement of these tests is currently being attempted by the use of MAbs together with the polymerase chain reaction (PCR). Epizootiological studies of fish diseases are beginning to emerge from the use of these reagents and techniques. In the near future these new methods should detect low levels of pathogens in adult carriers and perhaps in eggs, thus helping to prevent the dissemination of fish diseases. MAbs to fish pathogens are also being used for passive immunization studies as well as for conformational probes in the development of genetically engineered vaccines.

[Personal experience in the surgery of nasal sinus polyps]. / Experiencia personal en la cirugía de la poliposis nasosinusal.

Personal opinions on surgery of polyps of the nasal cavity and paranasal sinuses, including from simple polypectomy to ethmoid microsurgery and nasal endoscopic surgery. The diagnostic importance of tomodensitometry and the essential postsurgical care are emphasized. The results of 29 patients with polyps of the nasal cavity and paranasal sinuses treated with intranasal microsurgery and endoscopic surgery are reported.

Quantifying by monoclonal antibodies of specific IgG, IgM and IgA in the serum of minipigs experimentally infected with Actinobacillus pleuropneumoniae.

Fifteen minipigs were infected intratracheally with three different doses (10(8), 10(5) or 5 x 10(3) colony forming units) of the reference strains of serotypes 2 or 4 of Actinobacillus pleuropneumoniae, and three remained as controls. The titre of specific IgG, IgM and IgA in the serum was measured weekly for 15 weeks with an indirect ELISA using monoclonal antibodies specific for each isotype. IgG attained the highest titres, IgM lower and IgA the lowest, being only detected in four animals. Serotype 4 evoked significantly higher titres than serotype 2 (P < or = 0.01). In general the highest IgG titres were attained at four to six weeks after infection. Some of the minipigs were reinfected after seven weeks but this evoked an increased titre in only two instances.